Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged MEK2 mutants in Escherichia coli strain BL21(DE3) and in HEK-293T cells, GST pulldown assay on glutathione resin with recombinantly expressed FLAG-tagged E2 protein | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a stable PK-15 cell line with MEK2 knockdown by expression of specific shRNA | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of MEK2 by MEK1/ERK1/2-specific inhibitor PD98059 | Sus scrofa | |
U0126 | a specific inhibitor for MEK1/2/ERK1/2 | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + extracellular regulated kinase 1 | Sus scrofa | - |
ADP + phosphorylated extracellular regulated kinase 1 | - |
? | |
ATP + extracellular regulated kinase 2 | Sus scrofa | - |
ADP + phosphorylated extracellular regulated kinase 2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | I3L5D4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PK-15 cell | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + extracellular regulated kinase 1 | - |
Sus scrofa | ADP + phosphorylated extracellular regulated kinase 1 | - |
? | |
ATP + extracellular regulated kinase 2 | - |
Sus scrofa | ADP + phosphorylated extracellular regulated kinase 2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEK2 | - |
Sus scrofa |
mitogen-activated protein kinase kinase 2 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of MEK2 significantly promotes the replication of classical swine fever virus,CSFV, whereas knockdown of MEK2 by lentivirus-mediated small hairpin RNAs dramatically inhibits CSFV replication | Sus scrofa |
metabolism | mitogen-activated protein kinase kinase 2 (MEK2) is a kinase that operates immediately upstream of extracellular regulated kinase 1/2 (ERK1/2) and links to Raf and ERK via phosphorylation. The mitogen-activated protein kinase kinase/extracellular regulated kinase (MEK1/2/ERK1/2) cascade is involved in the replication of several members of the Flaviviridae family, including hepatitis C virus and dengue virus, or of classical swine fever virus, a psetivirus in swine | Sus scrofa |
additional information | MEK2 does not affect CSFV replication after blocking the interferon-induced Janus kinase signal transducer and activator of transcription (JAK-STAT) signaling pathway by ruxolitinib, a JAK-STAT-specific inhibitor. U0126 but not PD98059 inhibits CSFV replication | Sus scrofa |
physiological function | mitogen-activated protein kinase kinase 2, a novel E2-interacting protein, promotes the growth of classical swine fever virus, CSFV, via attenuation of the JAK-STAT signaling pathway. The MEK2-E2 interaction occurs during the late stage of CSFV replication and requires the C-termini of MEK2, residues 266 to 400, and E2 | Sus scrofa |